ABSTRACT
Objective Many studies showed that Ca~(2+) channel blocker could prevent and treat right ventricular hypertrophy(RVH) induced by chronic hypoxia.To further identify the mechanism,we investigated the effect of Ca~(2+) channel blocker on the levels of myocardial calcineurin A?mRNA(CnA?)in RV and plasma nitric oxide(NO),NO synthase and endothelin-1(ET-1) in rats with chronic hypoxia.Methods 30 rats were divided into three groups by randomized block design: treatment group with Amlodipine Besylate ablets [(30 mg?kg~(-1)?d~(-1)),administered via gavage],chronic hypoxia group,and control group.The rats in treatment group and chronic hypoxia group were exposed to normobaric chronic hypoxia [(10.0?0.5)% O_2 ] for 21 days.On the 21st day of experiment,all rats were sacrificed and the hearts were collected for measuring the weight.Blood samples were also drawn from the ventricles for measuring plasma NO,iNOS and ET-1 levels.CnA?mRNA levels in RV were measured by RT-PCR.Results ⑴The RV/(LV+S)、RV/BW ratios were significantly higher in chronic hypoxia group than those of control group and treatment group(P
ABSTRACT
To explore th e role of nitric oxide(NO), nitric oxide synthetase (NOS)and prolactin (PRL) in the pathogenesis,progress and prognosis of neonatal hypoxic-ischemia encephalop athy (HIE),plasma NO, NOS and serum PRL were determined by radioimmunoassay(RIA) and colorimetry in 68 newbom infants with HIE and 30 normal control neonates, r espectively. The results showed that the levels of NO, NOS and PRL were signific antly higher in all neonates with HIE than that in the control group (P＜0.05).There were in particular more higher levels of NO,NOS an d PRL in infants with either moderate or severe HIE(P＜0 .01). It was noticed that the level of PRL was much higher in HIE infants with seizures than that in HIE infants without seizures. There was a positive relati onship between the levels of PRL and the duration of seizure attack( P＜0.01). It is suggested that the levels of NO, NOS and PRL is ass ociated with HIE.The determination of these parameters will be valuable clinical ly for the diagnosis, judgement of prognosis and estimation of seizure attack in newborn infants with HIE.
ABSTRACT
Objective To observe the level of serum transforming growth factor ?(TGF ?) in patients with colorectal cancer and study its clinical significance. Methods Serum TGF ? was measured with radioimmunoassay in 54 patients with colorectal cancer and 25 healthy subjects,compared with 42 patients with colorectal cancer before and after the operation. Results The levels of serum TGF ? were significantly higher in patients with colorectal cancer than those in healthy controls(P